Proteomics investigations

Expertise is provided for the characterization of proteomic expression profile in human cells obtained from tissues of patients affected by high social impact diseases (muscular-skeletal, neurodegenerative and hematologic disorders) to enable the identification of early diagnosis and prognosis markers and potential therapeutic targets.

Proteomic expression profile characterization in human cells involved in high social impact diseases (muscular-skeletal, neurodegenerative and hematologic disorders) can allow the identification of early diagnosis and prognosis markers and potential therapeutic targets.
The RAMSES laboratory offers its know-how concerning protein biochemistry and isolation, characterization, identification and relative quantification methods.
In the laboratory the following tests are performed: protein extraction from cells, subcellular fractions (nuclei, mitochondria) and tissues; protein separation by polyacrylamide gel electrophoresis classical techniques; proteolytic peptides and phosphopeptides fractionation by HPLC. Protein identification is carried out by mass spectrometry LC-ESI-MS-MS. The data obtained are statistically validated through the use of specific licensed or open source available software.

Figure 16. Schematic representation of protein-protein interactions analysis steps carried out by the Affinity Purification Mass Spectrometry (AP-MS) functional proteomics approach

Content updated 13/02/2015 - 16:00
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