Regeneration of osteo-cartilagineous tissues

Skills related to isolation, growth, phenotypic and functional characterization and differentiation of cells from different types of human and animal tissue (mononuclear cells, mesenchymal cells of different origin like bone marrow and adipose tissue, chondrocytes, osteoblasts, synoviocytes, tenocytes, ligament cells, cells from dentin) and/or cell lines.

Regeneration of osteo-cartilagineous tissues
The laboratory offers activities and skills related to isolation, growth, phenotypic and functional characterization and differentiation of primary culture cells obtained from different types of human and animal tissues (mononuclear cells, mesenchymal cells of different origin such as bone marrow, adipose tissue etc., chondrocytes, osteoblasts, synoviocytes, tenocytes, ligament cells) and/or cell lines. These activities are mainly intended to cell characterization in the context of transplantation for osteo-cartilaginous tissue regeneration, to the definition of the molecular mechanisms underlying some joint diseases and to the execution of pre-clinical testing validating molecules with potential therapeutic relevance. The laboratory has the expertise and is equipped to prepare cell or tissue cultures under different experimental conditions: oxygen tension setting and mechanical stimuli imposition controlled by a bioreactor.

Figure 1. Histochemical analysis by means of Alizarin Red and Alcian Blue staining on monolayer culture concentrated bone marrow cells differentiated into osteogenic and chondrogenic sense. The red color indicates bone matrix production, the blue one shows the presence of cartilage matrix

Figure 2. IOR-G1 kit (IOR patent), indicated for the treatment of osteochondral defects

Figure 3. A bioreactor used to develop cell or tissue cultures in different experimental conditions: oxygen tension setting and controlled mechanical stimuli imposition

Content updated 13/02/2015 - 15:57
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