E-mail address: irene.dirignani@ior.it
Dr. Dirignani Irene
CV
Qualification
Biotechnologist
Current assignment
PhD student
Title of qualification awarded
2021: Master's Degree in Medical biotechnologies (final grade: 110/110 cum laude), University of Padua
Other qualifications awarded
- 2023: II level Master in omics data analysis (final grade: Excellent), University of Padua
- 2018: Bachelor's Degree in Biotechnology (final grade: 108/110), University of Padua
- 2022: Qualification to practice the profession of Biologist - section A
Positions held
- November 2023 – present: PhD student (Course of Oncology, Hematology and Pathology Unibo) at the Experimental Oncology laboratory of the Rizzoli Orthopedic Institute, Bologna.
- December 2021 – September 2023: Fellow at the Immunology Research Group, National Institute of Molecular Genetics (INGM)
- May 2021 – September 2021: Post-graduation Erasmus+ trainee at the Max Delbrück Center for Molecular Medicine (Berlin)
- March 2020 – March 2021: master's thesis internship at the Ludwig Cancer Research Institute (Lausanne)
- March - July 2018: three-year degree thesis internship at the laboratory of Professor Brini, department of Biology
Languages
Italian: netive speaker
Inglese: excellent (C1)
Francese: scholastic
Technologies
- Isolation of PBMCs from buffy coats and tumor infiltrates using ficoll
- Isolation of immune subpopulations (e.g., CD8+, CD4+ T lymphocytes, CD14+ monocytes) via MACS
- Isolation of immune subpopulations via FACS
- Flow cytometry (intra and extracellular staining)
- Cell cultures in suspension (CD8+, CD4+ T lymphocytes) and adhesion (HEK293, macrophages); co-culture of CD8+ T lymphocytes with APC
- Generation of CD8 T lymphocyte clones in Terasaki plates
- ELISpot
- Isolation and characterization of extracellular vesicles
- Molecular Biology Techniques: nucleic acid extraction and quantification (Nanodrop, Qubit); PCR; Real Time PCR; Reverse transcription; protein extraction; Western Blotting;
- 3D cultures in matrigel of colon and lung organoids and tumors
- Softwares: Adobe Acrobat / Microsoft Office (Microsoft) / GraphPad Prism / Snapgene viewer / Image lab software (Bio Rad) / BD FACSDiva™ & FlowJo Software / Adobe Photoshop / ImageJ (Fiji) / QuantStudioTM Design & Analysis Software / R - Programming
Scientific activities
Study and characterization of the immune infiltrate in different tumors with the aim of identifying new therapeutic approaches