Introduction
Morphological analysis
- inclusion, section and staining of histological preparations
- preparation of cytological samples
- immunohistochemical staining and image analysis.
Cell biology
- primary cultures from normal and pathological tissues, cultures of normal and transformed cell lines
- multiple separations of cell populations
- proliferative activity and cytolytic activity assays
- transfection of nucleic acids, stable transduction of interfering RNA
- immunoenzymatic reactions and dosages of soluble factors by multiplex technologies
- immunofluorescence staining, flow cytometry
- flow-fish.
Molecular biology
- PCR (polymerase chain reaction), RT-PCR (reverse transcription-PCR), real-time PCR
- genomic expression profiling, gene expression scanning
- western blot
- in situ hybridization.