Parra Alessandro, PhD
Biotechnologist, Biologist, PhD
Professional collaborator in health research
- Master Degree in Veterinary Biotechnology.
- Master Degree in Molecular and Cellular Biology.
National qualification as Biologist.
- From 2020: Research activity as a professional collaborator in health research at the Experimental Oncology Laboratory, Rizzoli Orthopaedic Institute.
- From 2014: Researcher at the Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute.
- 2002-13: Research and Molecular analyst at the Simple Department Structure of Medical Genetics and Rare Diseases, Rizzoli Orthopaedic Institute.
- French: excellent fluency in both written and spoken language.
- English: good fluency in both written and spoken language.
- German: sufficient fluency in both written and spoken language.
Extraction of nucleic acids from any biological matrix.
Extensive gene sequencing with Sanger technology (TP53, EXT1-2, genes of the collagen family and CD99).
Qualitative and quantitative analysis of genomic sequences, analysis of epigenetic methylation of DNA by mean of bisulfite conversion, analysis of genomic rearrangements by MLPA-MAPH, real-time PCR with SYBR Green I Dye and new generation dyes, high skills in HRM (High Resolution Melting) set up and analysis, extraction of glycosylated proteins and glycosaminoglycans in native form from extracellular matrices and subsequent analysis in liquid phase NMR, in situ hybridization of radioactive riboprobes.
Overall basic knowledge of a genetics and molecular biology laboratory. Ability to set up cell cultures and organotypic ones. Use of common equipment in molecular biology and genetics laboratory; in particular automated sequencer (ABI Prism 3100, Applied Biosystems), dHPLC (Wave 3500 HT, Transgenomic) and Real Time PCR machines (ABI 7900HT and ViiA7 (Life Technologies) and Rotorgene 6000 (Corbett). Refined theoretical knowledge of the most important Next Generation Sequencing platforms (Illumina, pyrosequencing by Roche, SMRT Pacific Biosciences, cPAL, Solid and Ion Torrent/Proton).
Practical knowledge of the Illumina NextSeq500 platform: creation of libraries for RNA-Seq and fusion genes detection.
Supervisor of theses in Molecular and Cellular Biology (year 2013 and 2017).
Substitute teacher for six years for the course of Medical genetics for Podiatrists and Orthopedic Technicians.
Author of 8 scientific articles and participation to several congresses.
Significant contributions to research projects: 2006-2011: European Union "EuroBoNet, European Network to Promote Research into Uncommon Cancers in Adults and Children: Pathology, Biology and Genetics of Bone Tumours".
Analysis of proteoglycans and glycosaminoglycans extracted from cartilage extracellular matrix and genetic analysis of several genes of interest regarding tumor progression in Ewing's Sarcoma and Osteosarcoma. Next Generation Sequencing for molecular discovery of driver factors in Sarcomas.