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RAMSES Laboratory

Introduction

The RAMSES laboratory carries out biomedical research activities concerning the muscular-skeletal system.

In particular, preclinical evaluations are performed in the field of tissue engineering and rheumatic diseases, such as osteoarthritis, with the aim to set up possible applications/industrial developments.

Data interpretation with the FACS (Fluorescent-Activated Cell Sorter) instrument, a computerized system that analyzes samples of cells labeled with monoclonal antibodies.

The laboratory makes available industrial research and technological innovation activities such as:

  • Development and validation of medical devices, biological compounds, and cell therapies
  • Biofabrication
  • Pharmaceutical and Nutraceutical
  • Biomarkers.

The laboratory is also able to provide know-how for the design, drafting, reporting of research projects on a national, international and industrial basis.

Please download our informative brochure with a brief description of the Laboratory Ramses services.

Research Activity

Research Activity
Development and validation of medical devices, biological compounds and cell therapies

Several lines of research are active in the laboratory, aimed at the characterization and evaluation of the clinical potential of: medical devices such as visco-supplementation products; biological compounds such as platelet rich plasma (PRP), bone marrow concentrate and adipose tissue concentrate; cells isolated from various sources, alone or in combination with biomaterials supplied externally or made by the laboratory itself by 3D printing. Primary monolayer cultures are obtained from different types of human and animal tissue (mononuclear cells, mesenchymal cells of different derivation such as bone marrow, adipose tissue, bone tissue etc., chondrocytes, osteoblasts, synoviocytes, tenocytes, ligament cells) and subsequently characterized from a proliferative, phenotypic and functional point of view, both in basal conditions and inducing differentiation in chondrogenesis, osteogenesis, etc.. Cellular and/or tissue cultures are prepared even under experimental conditions different from those shown: in tri-dimension, in pellets, under regulated oxygen tension conditions and under controlled mechanical stimulations through the use of a bioreactor. The preclinical optimization of the constructs can be performed both in static and dynamic systems (e.g. Flexercell and Bioreactors). Structural and morphological evaluations are also performed through histological analysis and evaluations in optical microscopy and image analysis. These activities are aimed at the evaluation of tissues engineered for transplantation for the regeneration of osteo-cartilaginous tissues, the definition of molecular mechanisms underlying some joint diseases and the performance of preclinical tests for the validation of molecules with potential therapeutic relevance.

 

Figure 1: Preparation of a cell culture; characterization; monolayer or 3D culture under static or dynamic (bioreactor) conditions; functional assays.

 

Figure 2: Characterization of biological compounds (PRP, PRF, APS) and their activity on cell cultures (chondrocytes, synoviocytes, mesenchymal cells)

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Research Activity
Biofabrication

The laboratory offers a "customized" printing service with biomimetic materials of various nature through the combination of multiple printing technologies in a single process, in a controlled sterile environment. The present platform consists of two instruments:

  • 3D Discovery, a printer that builds the devices through the polymerization of cellularized or non-cellularized hydrogels, substances able to support the growth of various cell types, or the use of thermoplastic polymers for the realization of shelves and 3D models
  • 3D Discovery Evolution, which, in addition to the already mentioned technologies, allows the realization of constructs with characteristic sub-micro/nanometric dimensions through the use of electrospinning/writing technology both for casting and in solution.

Figura 3: Piattaforma di 3D Bioprinting. I dati dei pazienti sono ottenuti tramite immagini TAC effettuate presso la Radiologia dell'Istituto e processati mediante specifici software. I files sono inviati alle stampanti che realizzano il costrutto

Figura 4: Esempi di biofabbricazione. La tecnica permette di ingegnerizzare tessuti complessi utilizzando le cellule del paziente stesso (Biofabrication), con precisione micrometrica nel posizionamento cellulare

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Research Activity
Pharmaceutical and Nutraceutical

The RAMSES laboratory provides in vitro preclinical evaluations for the characterization of plant origin (nutraceuticals) and synthetic products, and their role as molecules potentially useful for the therapy of musculoskeletal diseases, such as osteoarthritis or osteoporosis. The skills and instrumentation present in the laboratory allow to acquire preliminary information on the effect and mechanism of action of natural or synthetic molecules, using advanced methodologies. The aim is to provide companies operating in the field of health food products (such as food supplements, foods for special medical purposes, enriched foods and, more generally, functional foods) or pharmaceutical industries, with services aimed at developing new products, improving their quality and guaranteeing, on a robust scientific basis, their safety and effectiveness, in accordance with the reference legislation. The expertise of researchers related to the Laboratory guarantees total coverage of the necessary skills for research and development of innovative and high quality products, whose properties are certified by studies conducted with high methodological rigor, following the regulations in force and the guidelines issued by the main institutions operating in the field of nutraceutical and pharmaceutical products and quality certification. In the pharmaceutical field, a family of molecules capable of stimulating the anabolic function of bone tissue has been developed and patented (PCT/IB2015/058551 "New molecules for bone regeneration").

Figura 5: Terapie farmacologiche innovative per il trattamento di patologie dell'apparato muscolo-scheletrico. Studio di nuove molecole in grado di stimolare il metabolismo osseo utili per contrastare le fragilità di questo tessuto

 

Figura 6: Screening di molecole nutraceutiche con potenziale attività sul metabolismo osseo

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Research Activity
Biomarkers

In the laboratory are active various research activities aimed at identifying markers of early diagnosis, prognosis and potential therapeutic targets and progression of some osteoarticular pathologies. To this end, phenotypic and functional characterization tests are performed on cell populations involved in the development of various diseases of the musculoskeletal system; studies to analyze the molecular mechanisms involved in the pathogenesis or response to therapies. In the laboratory methods of protein isolation, characterization, identification and quantification are applied. In particular, we perform protein extraction from cells and tissues, separation of proteins by classic techniques of polyacrylamide gel electrophoresis. Functional evaluations are performed through the analysis of soluble factors, such as cytokines, chemokines and growth factors, released at the joint level.

 

Figure 7: Study of biomarkers of bone metabolism. Role of hydrogen sulfide

Figure 8: Identification of biomarkers of activity and progression of musculoskeletal disorders

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Attachments

Documents and videos about the ward/service

Documents and videos about the laboratory

Contacts and Locations

Contacts

Contact:

Secretary's office
phone +39-051-6366803
fax +39-051-6366807
e-mail ramses@ior.it
 

Where are our locations?

Researche centre Codivilla-Putti

Istituto Ortopedico Rizzoli

via di Barbiano 1/10

40136 Bologna (Italy)

Patents

Brevetti del personale dell'unità operativa.

Patent
Composition containing autologous mononuclear cells and pig collagen scaffold in paste form and its use in the preparation of a medicament for surgical treatment
Composizione comprendente cellule mononucleate autologhe supportate su matrice scelta dal gruppo comprendente matrice liofilizzata o matrice precostituita sottoforma di membrana o tessuto, con eventuale aggiunta di gel piastrinico, nonché uso di detta composizione per la preparazione di medicamenti per trattamenti chirurgici.
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Training events

Personale della struttura ha promosso i seguenti eventi formativi.

Immagine locandina ICRS Focus Meeting (particolare)
ICRS Focus Meeting Ortho-Biologics
From the Laboratory to the Clinics: New Insight into Translational Research toward Bio-Orthopaedics
from  
Thursday - 6 October 2022
to  
Friday - 7 October 2022
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